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2020 IPS Conference
Study Materials
Corporate Members
Home
About/Contact
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Events/Seminars
2020 IPS Conference
Study Materials
Corporate Members
Spectroscopic properties of cationic dye Basic Orange 21 (BO21) in solutions, in solids and within leukocytes were examined. Results obtained with solutions indicate that influence of variables like pH, viscosity, salt composition and various proteins on the absorption spectrum of BO21 is negligible. However, in the presence of heparin, a blue shift (484nm-465nm) is observed, which is attributed to aggregation of BO21 on the polyanion. However, when embedded in basophils, the absorption spectra of intracellular BO21 is extremely red shifted, with two peaks (at 505nm and 550nm), found to be attributed to BO21 and BO21-heparin complex respectively, which are intracellularly hosted in non-aqueous environments.