In recent days, the millimeter waves (MMWs) are widely used for biomedical applications. Previously, we have shown that the MMW affects the cancer cells precisely without generating similar results in non-cancerous cells. The BY4741 wild type (WT) Saccharomyces cerevisiae yeast cells were used for irradiation. Cells were grown overnight at 30°C in standard synthetic complete (SC) liquid medium for 16 hours. The yeast cells were spotted/dappled on the Agar medium and subsequently irradiated from the top. The grown cells were exposed to specific frequencies of 75 GHz, 85 GHz, 95 GHz, and 105 GHz with an amplitude of 5 dBm for 6 hours. The irradiation experiment for each frequency was repeated six times.

Primary observations suggested that the MMW (75-110 GHz) irradiations decrease the growth rate of yeast cells. The observed effect of the upregulation of cell division reported here cannot be accounted for the genetic mutation(s) caused due to MMW irradiation. Earlier studies involving MMW irradiation over 24 hours reported no DNA damage or strand break due to such treatment. Biomolecules are known to undergo conformational vibrations upon MMW irradiation.  These vibrations are originated from the displacement of electric charges on their surface. This indicates that MMW irradiations possibly interact with the proteome responsible for cell division in yeast, pertaining to the current study. These findings allow us to use this technology to identify novel protein-protein interactions using non-invasive MM wave irradiation and study their corresponding cellular functions.